Asymmetric Transcription of Deoxyribonucleic Acid by Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase of Krebs II Ascites-Tumour Cells
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Deoxyribonucleic acid-dependent ribonucleic acid polymerase of Caulobacter crescentus.
Deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) was purified from the dimorphic bacterium Caulobacter crescentus at three stages in development. Enzyme from pure populations of stalked cells, as well as populations enriched in swarmer and predivisional cells, appeared identical in subunit structure and template requirements. The molecular weights of the enzyme sub...
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Highly purified trachoma elementary bodies (T'ang strain), incubated in the presence of the four nucleoside triphosphates [Mg(2+), Mn(2+), 2-mercaptoethanol, tris(hydroxymethyl)aminomethane buffer (pH 7.5)] were found to incorporate (3)H-uridine triphosphate (UTP) into ribonucleic acid (RNA) molecules. Eighty-seven per cent of the labeled molecules were sensitive to ribonuclease treatment. In v...
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The synthesis of DNA complementary to rabbit globin mRNA by the enzyme RNA-directed DNA polymerase (from avian myeloblastosis virus) has been studied. The reaction is dependent on mRNA as template, oligo(dT) as primer, and the four deoxynucleoside triphosphates as substrates. The 28 S, 18 S, and 4 S RNA are not effective templates; oligo(dG), oligo(dC), and oligo(dA) are not effective primers. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1966
ISSN: 0006-2936
DOI: 10.1042/bj0980005c